office (978) 922-9446
  fax (978) 922-1666
info@soltecventures.com

"We React"

Protein Modification Reagents                      TCEP

 

Economically equivalent, odor free reductions at DTT prices available in large quantities with very high purity and stability. In addition Soltec Ventures offers a TCEP stable solution. The solution is 0.5M and stable at room temperature years under inert atmosphere.

Unit Price
1gm $18.00
Data not available (103)

Return to Previous Page
'"> 2gm $32.00
Data not available (103)

Return to Previous Page
'">
 5gm $65.00
Data not available (103)

Return to Previous Page
'">
 10gm $105.00
Data not available (103)

Return to Previous Page
'">
 25gm $230.00
Data not available (103)

Return to Previous Page
'">

M115     Tris(2-carboxyethyl)phosphine hydrochloride, TCEP

                                     f.w.: 286.6daltons     [ 51805-45-9 ]

Stability!
  • Odorless and non-volatile
  • Solid is stable in air for several months
  • Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH<7.66

Solubility!

    310g/l in water

Reactivity!
  • TCEP reduces organic disulfides to thiols rapidly and quantitatively in water
  • More complete reductions than 1,4-dithiothreitol ( DTT ), Cleland's reagent
  • Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.
  • Dilute (1mM) TCEP solutions react rapidly at room temperature
  • The strength of the phosphorus-oxygen bond makes the reaction irreversible
  • Kinetics rather than thermodynamics controls the reduction

Selectivity!

  • TCEP is selective for disulfides
  • TCEP does not react with other functional groups on proteins
  • Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations

TCEP References:

    1. Kirkley, T.L., Anal. Biochem, 180, 231-236 (1989).

    2. Andrews, P. C., Dixon, J.E., Anal. Biochem, 161, 524-528 (1987).

    3. Schonberg, A., Chem Ber., 163-164 (1935).

    4. Rauhut, M., et. al., JACS, 81, 1103-1107 (1959).

    5. Chen, C.S., Fujimoto, Y., Girdoukas, G.Sih, C.J., JACS, 109, 6825-6309 (1987).

    6. Houk, J., Whitesides, G.M., JACS, 109, 6825-6836 (1987).

    7. Grayson, M., Farley, C.E., Chimie Organique du Phophore, Collogues Int'l du Centre Nat'l de la Recherche Scientifique, No. 182  CNRS, Paris, 275-284 (1970).

    8. Overman, L.E., O'Conner, E.M., JACS, 98, 771-775 (1977).

    9. Rosenfield, R.E., Parthasarthy, R., Dunitis, J.D., JACS, 99, 4860-4862 (1977).