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BCA Protein Assay

 

Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid ( BCA ) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu⁺² protein complex under alkaline conditions, followed by reduction of the Cu⁺² to Cu⁺¹. The amount of reduction is proportional to the protein present. It has been shown that cysteine, trytophan, tyrosine and the peptide bond are able to reduce Cu⁺² to Cu⁺¹. BCA - bicinchoninic acid forms a purple blue complex with Cu⁺¹ in alkaline solutions. This provides a basis to monitor the reduction of alkaline Cu+2 by proteins and thus determine the protein concentration in a biochemical sample. Bicinchoninic acid is a highly chromogenic reagent when complexed with Cu⁺¹, forming a purple blue complex with an absorbance maximum  at 562 nanometers. The absorbance is directly proportional to the protein concentration.

• BCA Cu⁺¹ color complex is stable
• Little susceptibility to detergents ( see compatibility chart )
• BCA is applicable over a broad range of protein concentrations

  stock #	unit	price
  BCA 250	250ml	$30.00
  BCA 500	500ml	$55.00
  BCA 1000	1000ml	$80.00
  BCA 3750	3.75liter	$300.00
  BCA 25	25gm	$240.00
  BCA 250 kit	1250 determinations	$42.00
  BCA 500 kit	2500 determinations	$75.00
  BCA 1000 kit	5000 determinations	$95.00
  BCA gal kit	18000 determinations	$340.00

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

BCA References:

    1. Smith, P.K., et al, Anal. Biochem., Measurement of Protein Using Bicinchoninic Acid, 150, 76-85 (1985)